The Art of Inducing Hypoxia

Many cells in the human body strongly react on decreased oxygen concentrations, generally defined as hypoxia. Therefore, inducing hypoxia vitro is essential for research. Classically, induced using a chamber, but alternative methods exist that do not require special equipment. Here, we compared three different to induce without chamber: chemical stabilization of HIF-1? by CoCl2, decrease pericellular concentrations increased media height, and consumption an enzymatic system. Hypoxia induction was further analyzed within cell culture systems: 2D (adherent) osteoprogenitor cells, monocytic (suspension) 3D fracture hematoma model. The were scope healing regarding inflammation differentiation. We could show all feasible adherent cells. Increased heights did stimulate hypoxic response suspension Chemical showed limitations when looking at expression cytokines osteoprogenitors monocytes. Enzymatic reduction proofed be most effective systems